摘要： 目的·探討妊娠期糖尿病（gestational diabetes mellitus，GDM）孕婦與健康孕婦之間腸道菌群的差異。方法·招募2017年8月—2019年1月于上海交通大學(xué)醫(yī)學(xué)院附屬新華醫(yī)院產(chǎn)科產(chǎn)檢孕婦74例，其中GDM孕婦（GDM組）51例、健康孕婦（健康組）23例，留取所有孕婦的新鮮糞便樣本。利用Illumina MiSeq平臺(tái)對(duì)2組樣本細(xì)菌的基因序列進(jìn)行高通量測(cè)序，再采用線性判別分析（linear discriminant analysis，LDA）、組間群落差異分析（LDA EffectSize，LEfSe）分別對(duì)2組糞便樣本的菌群進(jìn)行腸道菌群α多樣性及表達(dá)豐度差異檢驗(yàn)。結(jié)果·與健康組相比，GDM組孕婦的腸道菌群豐度更高（P0.027），在科級(jí)別上紫單胞菌科（Porphyromonadaceae）、產(chǎn)堿菌科（Alcaligenaceae）豐度更高，在屬級(jí)別上擬桿菌屬（Bacteroides）豐度更高（均PNeisseria）、鏈球菌屬（Streptococcus）、產(chǎn)丁酸菌（Butyricicoccus）、加德納菌屬（Gardnerella）、奇異菌屬（Atopobium）豐度更高（均P結(jié)論·GDM孕婦的腸道菌群與健康孕婦間存在較為明顯的差異。

關(guān)鍵詞: 妊娠期糖尿病, 腸道菌群, 高通量測(cè)序, &, alpha, 多樣性

Abstract:

Objective · To investigate the differences of intestinal flora between gestational diabetes mellitus (GDM) pregnant women and healthy pregnant women. Methods · In this study, 74 pregnant women the Department of Obstetrics in Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Aug. 2017 to Jan. 2019 were recruited, including 51 GDM pregnant women (GDM group) and 23 healthy pregnant women (healthy group). High-throughput sequencing was performed on bacterial gene sequences the fresh feces of all pregnant womenusing Illumina MiSeq platform. Linear discriminant analysis (LDA) and LDA EffectSize (LEfSe) were used to detect the α diversity and abundance of intestinal flora of the two groups. Results · Compared with the healthy group, the intestinal flora richness of pregnant women in the GDM group was significantly higher (P0.027). Porphyromonadaceae and Alcaligenaceae in Family, and Bacteroides in Genus of microbiota in the GDM group were significantly higher (all P in Order, Lachnospirace in Family, and Neisseria, Streptococcus, Butyricicoccusm, Gardnerella and Atopobium in Genus of microbiota in the healthy group were significantly higher (all PConclusion · There are significant differences of intestinal flora between GDM pregnant women and healthy pregnant women.

Key words: gestational diabetes mellitus (GDM), intestinal flora, high-throughput sequencing, &alpha, diversity

中圖分類號(hào): 

R714.7

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