摘要： 目的 挖掘藏醫(yī)治療朗秀病(濕疹)的經(jīng)典方藥,并對應(yīng)用廣、療效好的名方五味姜黃膏的經(jīng)典理論進(jìn)行梳理完善,考證其對濕疹小鼠模型的干預(yù)作用,分析其對家兔皮膚的急性毒性影響。方法 取 BALB/c 小鼠50只,隨機(jī)選取10只作為空白組,其余40只制備小鼠濕疹模型。用7%高濃度DNCB丙酮橄欖油溶液致敏2次,再用0.5%低濃度DNCB丙酮橄欖油溶液20 μL激發(fā)1次,觀察小鼠背部皮膚出現(xiàn)紅斑、水腫及滲出液等急性皮炎現(xiàn)象。造模成功后,將40只濕疹小鼠按隨機(jī)數(shù)字表法分成4組,分別是模型組、丁酸氫化可的松乳膏組、五味姜黃膏高劑量組、五味姜黃膏低劑量組,每組10只。除模型組外,其余3組分別給予相應(yīng)藥物外涂干預(yù) 36 d,確定藥物療效。另取新西蘭家兔16只,隨機(jī)數(shù)字表法將其分為4組,造模,涂藥。連續(xù)觀察14 d,每日觀察2次,觀察其安全性。結(jié)果 對各組小鼠背部皮膚采用盲法計(jì)分,五味姜黃膏高、低劑量對小鼠背部皮損組織有良好的愈合作用。與模型組比較,五味姜黃膏高、低劑量和丁酸氫化可的松乳膏在降低第 15、30天皮損積分(P<0.01)、降低小鼠耳腫脹厚度(P<0.01)、降低小鼠脾臟指數(shù)(P<0.01)方面,差異有統(tǒng)計(jì)學(xué)意義。與模型組比較,五味姜黃膏高、低劑量和丁酸氫化可的松乳膏在降低小鼠血清 TNF-α水平方面,差異有統(tǒng)計(jì)學(xué)意義(P<0.01)。五味姜黃膏高、低劑量可降低小鼠血清IL-6,但差異無統(tǒng)計(jì)學(xué)意義(P>0.05)。家兔毒性實(shí)驗(yàn)結(jié)果表明,五味姜黃膏對家兔局部皮膚無明顯損傷。結(jié)論 在對比五味姜黃膏與丁酸氫化可的松乳膏陽性對照研究后,明確了五味姜黃膏治療朗秀病(濕疹)療效僅次于丁酸氫化可的松乳膏,證實(shí)了藏醫(yī)古籍中五味姜黃膏對朗秀病(濕疹)的治療作用。五味姜黃膏對正常皮膚、破損皮膚都可以按正常劑量使用。

關(guān)鍵詞: 藏醫(yī)朗秀病, 五味姜黃膏, 小鼠濕疹模型, 丁酸氫化可的松, DNCB致敏

Abstract: Objective To explore the classic prescriptions of Tibetan medicine for the treatment of Langxiu disease(eczema), and sort out and improve the classic theory of the famous prescription Wuwei Jianghuang ointment which is widely used and has good curative effects, and examines its effects on the eczema mouse model, and analyzes and evaluates its effects on the eczema mouse model, then acute toxic effects on the skin. Methods Fifty BALB/c mice,10 mice were randomly selected as blank group, and the other 40 mice were prepared to model eczema. The mice were sensitized twice with 7% high concentration DNCB acetone olive oil solution, and then stimulated once with 0.5% low concentration DNCB acetone olive oil solution 20 μL. Acute dermatitis such as erythema, edema and exudation of back skin were observed. After successful modeling, 40 eczema mice were randomly divided into 4 groups, which were model group,the hydrocortisone butyrate cream group Wuwei Jianghuang ointment high-dose group and Wuwei Jianghuang ointment low-dose group, with 10 mice in each group. Except the model group, the other 3 groups were treated with corresponding drugs for 36 days, respectively, to determine the drug efficacy. Another 16 New Zealand rabbits were selected and divided into 4 groups by random number table method. Continuous observation went on for 14d, 2 times a day. Results Blind scoring of the back skin of mice in each group found that the high and low dose groups of Wuwei Jianghuang ointment had a good healing effect on the back skin lesions of mice. Compared with the model group, the high-dose and low-dose Wuwei Jianghuang ointment groups and the hydrocortisone butyrate cream group had statistical significance in the skin lesion scores on the 15th and 30th days(P<0.01), and had statistical significance in the ear swelling thickness(P<0.01). And had statistical significance in the spleen index of mice(P<0.01). Compared with the model group, the high and lowdose groups of Wuwei Jianghuang ointment and hydrocortisone butyrate cream groups had statistical significance in reducing the serum TNF-α level of mice(P<0.01). There were no statistical significance in decreasing serum IL-6 in mice between the high and low dose groups of Wuwei Jianghuang ointmenthad(P>0.05). Wuwei Jianghuang ointment had no obvious toxicity. Conclusion After the positive control study of Wuwei Jianghuang ointment and hydrocortisone butyrate ointment, the curative effects of Wuwei Jianghuang ointment in the treatment of Langxiu disease(eczema)is confirmed to be second only to hydrocortisone butyrate ointment, and the efficacy of Wuwei Jianghuang ointment in ancient Tibetan medicine books on the treatment of Langxiu disease(eczema)is confirmed too. Wuwei Jianghuang ointment can be used in normal dosage for normal skin and damaged skin.

Key words: Tibetan Medicine Langxiu Disease, Wuwei Jianghuang ointment, mice model of eczema, hydrocortisone butyrate, DNCB sensitization

中圖分類號: 

R285.5

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網(wǎng)址: 藏藥五味姜黃膏藥效學(xué)及急性毒性分析 http://www.u1s5d6.cn/newsview849841.html