摘要: 為研究不同產(chǎn)地黃芪的化學(xué)成分差異，制備了甘肅和內(nèi)蒙古2個產(chǎn)地共15批黃芪藥材的水煎液，利用超快速液相色譜-離子阱飛行時間質(zhì)譜儀(UFLC-IT-TOF/MS)采集15批黃芪藥材水煎液化學(xué)成分的指紋圖譜，進而運用偏最小二乘-判別分析(PLS-DA)和(非)參數(shù)檢驗對數(shù)據(jù)進行模式識別和統(tǒng)計分析，以變量投影重要性大于1、統(tǒng)計學(xué)差異小于0.05以及倍數(shù)變化大于1.5為閾值，篩選并鑒定其差異成分。結(jié)果發(fā)現(xiàn)，不同產(chǎn)地的黃芪水煎液中毛蕊異黃酮、芒柄花素、亞油酸等7個化學(xué)成分的含量有明顯差異，涉及木脂素、黃酮、脂肪酸等4種類別。本研究為黃芪藥材的全面質(zhì)量控制、準確區(qū)分產(chǎn)地以及合理種植栽培提供了重要依據(jù)。

Abstract: To uncover the differences in material basis of Astragalus membranaceus from different origins, ultra-fast liquid chromatography-ion trap time-of-flight mass spectrometry(UFLC-IT-TOF/MS)was applied to characterize chemical features of 15 batches of water decoction of Astragalus membranaceus from Gansu Province and Inner Mongolia Autonomous Region. Pattern recognition and statistical analysis were performed using partial least squares-discriminant analysis(PLS-DA)and(non)parametric tests, respectively. The differential compounds with variable importance in projection(VIP)> 1, adjusted P< 0. 05, and fold change(FC)> 1. 5 were screened out and further identified. It was found that 7 different substances such as calycosin, formononetin and linoleic acid could be used as quality markers to distinguish the origin of Astragalus membranaceus. Our findings provided new potential quality markers for the accurate differentiation of geographical origins of Astragalus membranaceus as well as its comprehensive quality control.

[1] Chinese Pharmacopoeia Committee.Pharmacopoeia of the People′s Republic of China:part 1(中華人民共和國藥典:一部)[S].Beijing:China Medical Science Press,2015:302-303. [2] Fu J,Wang Z,Huang L,et al.Review of the botanical characteristics,phytochemistry,and pharmacology of Astragalus membranaceus(Huangqi)[J].Phytother Res,2014,28(9):1275-1283. [3] Jin M,Zhao K,Huang Q,et al.Structural features and biological activities of the polysaccharides from Astragalus membranaceus[J].Int J Biol Macromol,2014,64:257-266. [4] Li M,Wang W,Xue J,et al.Meta-analysis of the clinical value of Astragalus membranaceus in diabetic nephropathy[J].J Ethnopharmacol,2011,133(2):412-419. [5] Jiao J,Gai QY,Niu LL,et al.Enhanced production of two bioactive isoflavone aglycones in Astragalus membranaceus hairy root cultures by combining deglycosylation and elicitation of immobilized edible Aspergillus niger [J].J Agric Food Chem,2017,65(41):9078-9086. [6] Shahzad M,Shabbir A,Wojcikowski K,et al.The antioxidant effects of Radix Astragali(Astragalus membranaceus and related species)in protecting tissues from injury and disease[J].Curr Drug Tar,2016,17(12):1331-1340. [7] Liao XF,Bi XL,Xu AL,et al.Fingerprint and three constituents′ quantitative determination for Astragali Radix from different regions by HPLC-DAD-ELSD[J].J Guangdong Pharm Univ(廣東藥科大學(xué)學(xué)報),2018,34(5):558-563. [8] Tian D,Li ZY,Fan SC,et al.NMR-based analysis of water soluble extracts of different Astragali Radix[J].Acta Pharm Sin(藥學(xué)學(xué)報),2014,49(1):89-94. [9] He JX,Mou QQ,Zhang JQ,et al.Rapid identification of Astragali Radix from different origins by UPLC combined with chemometrics methods [J].Chin Tradit Herbal Drugs(中草藥),2017,48(1):179-184. [10] Lai PK,Chan JY,Cheng L,et al.Isolation of anti-inflammatory fractions and compounds from the root of Astragalus membranaceus[J].Phytother Res,2013,27(4):581-587. [11] Li J, Li F, Xu Y, et al. Chemical composition and synergistic antioxidant activities of essential oils from Atractylodes macrocephala and Astragalus membranaceus[J].Nat Prod Commun,2013,8(9):1321-1324. [12] Guan QX, Li CD. Screening test study on seedling pattern of Astragalus membranaceus in different climate regions of Longxi(隴西不同氣候區(qū)域黃芪育苗模式篩選試驗研究)[J].J Chin Med Mate(中藥材),2016,39(3):490-492. [13] Liu DW,Gu CM,Yang QZ,et al.Resource surveys and suitability of origin for genuine medicinal materials,Astragalus membranaceus var.mongholicus in Inner Mongolia,China[J].Chin J Appl Ecol(應(yīng)用生態(tài)學(xué)報),2016,27(3):838-844. [14] Kai Z,Michela P,Antonio P and Annamaria P.Biological active ingredients of traditional Chinese herb Astragalus membranaceus on treatment of diabetes:a systematic review[J].Mini-Rev Med Chem,2015,15(4):315-329. [15] Zhang LJ,Liu HK,Hsiao PC,et al.New isoflavonoid glycosides and related constituents from astragali radix(Astragalus membranaceus)and their inhibitory activity on nitric oxide production[J].J Agric Food Chem,2011,59(4):1131-1137. [16] Nie Y,Li S,Yi Y,et al.Effects of astragalus injection on the TGFβ/Smad pathway in the kidney in type 2 diabetic mice[J].BMC Complem Altern Med,2014,14(1):148. [17] Li W,Sun YN,Yan XT,et al.Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells[J].Arch Pharm Res,2014,37(2):186-192. [18] Zhou R,Xu L,Ye M,et al.Formononetin inhibits migration and invasion of MDA-MB-231 and 4T1 breast cancer cells by suppressing MMP-2 and MMP-9 through PI3K/AKT signaling pathways[J].Horm Metab Res,2014,46(11):753-760. [19] Capozzi V,Menga V,Digesu AM,et al.Biotechnological production of vitamin B2-enriched bread and pasta[J].J Agric Food Chem,2011,59(14):8013-8020. [20] Yang B,Chen H,Stanton C,et al.Review of the roles of conjugated linoleic acid in health and disease[J].J Funct Foods,2015,15:314-325. [21] Villacorta L,Minarrieta L,Salvatore SR,et al.In situ generation,metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation[J].Redox Bio,2018,15:522-531. [22] Wang Y,Fan X,Qu H,et al.Strategies and techniques for multi-component drug design from medicinal herbs and traditional Chinese medicine[J].Curr Top Med Chem,2012,12(12):1356-1362. [23] Fu S,Gu Y,Jiang JQ,et al.Calycosin-7-O-β-d-glucoside regulates nitric oxide/caveolin-1/matrix metalloproteinases pathway and protects blood-brain barrier integrity in experimental cerebral ischemia-reperfusion injury[J].J Ethnopharmacol,2014,155(1):692-701.

相關(guān)知識

基于Cu
基于信息
無限極：基于“100
基于健康賦權(quán)的NNN
關(guān)于基因檢測的基本了解和普及（1）
基于STM32的健康監(jiān)測系統(tǒng)
基于PaddleSpeech的嬰兒啼哭識別(上）
關(guān)于甲殼素片的基本信息介紹
關(guān)于減肥的基本原理和飲食的基本原則，想瘦必知
基于SWOT的“饑餓營銷”策略探究.doc

網(wǎng)址: 基于UFLC http://www.u1s5d6.cn/newsview581284.html