基于廣泛靶向代謝組學(xué)分析黑老虎不同部位成分差異

基金項(xiàng)目:

貴州省科技計(jì)劃重大專項(xiàng)(黔科合平臺(tái)人才[2017]5411號(hào))；國家重點(diǎn)研發(fā)計(jì)劃課題(2016YFC0502601)資助

Metabolic in Different Tissues of Kadsura coccinea by Using Widely-targeted Metabolomics

Author:

GAO Jianfei

Guizhou Mountainous Resources Institute, Guizhou Academy of Sciences, Guiyang 55001, China
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YANG Yan

Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China
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摘要:

為提高黑老虎(Kadsura coccinea)資源的綜合利用率，采用廣泛靶向代謝組學(xué)技術(shù)鑒定并分析了根、莖、葉代謝組分差異及高度富集成分。結(jié)果表明，在根、莖和葉中分別鑒定出642、650和619個(gè)代謝物，以酚酸、脂質(zhì)、類黃酮和有機(jī)酸為主；葉與根、莖與根的共有成分分別為566和650個(gè)，顯著差異成分有442和393個(gè)，主要為酚酸、類黃酮和脂質(zhì)，差異代謝物在苯丙烷生物合成、黃酮與黃酮醇生物合成通路中顯著富集。代謝物總豐度和次生代謝物豐度均表現(xiàn)為葉 > 根 > 莖，葉中酚酸、類黃酮和脂質(zhì)及莖中酚酸積累量顯著高于根，而氨基酸及其衍生物、萜類、木脂素、香豆素、生物堿的豐度在根中顯著上調(diào)。因此，黑老虎根、莖、葉有大量共有成分，葉和莖中酚酸、葉中類黃酮和脂質(zhì)高度富集，含有新綠原酸、綠原酸、槲皮素等多個(gè)豐度較高且具有重要生物活性化合物，具有較高利用價(jià)值。

Abstract:

To improve the comprehensive utilization of Kadsura coccinea resources, the differential and highly enriched metabolic components of root, stem and leaf were analyzed by using wide range of targeted metabolomics techniques. The results showed that there were 642, 650 and 619 metabolites identified from roots, stems and leaves, respectively, in which phenolic acids, lipids, flavonoids and organic acids were dominant in quantity. There were 566 and 650 common metabolites in leaves and roots, stems and roots, respectively, of which 442 and 393 were significantly different metabolites, mainly including phenolic acids, flavonoids and lipids. Moreover, these differentially metabolites were significantly enriched in pathways of phenylpropane biosynthesis, flavone and flavonol biosynthesis. The total and secondary metabolites abundances were in the order of leaf > root > stem. The accumulation of phenolic acids, flavonoids and lipids in leaves and phenolic acids in stems were significantly higher than those in roots; while the abundance of amino acids and their derivatives, terpenoids, lignans, coumarins and alkaloids were significantly up-regulated in roots. Therefore, there were a large number of common components in roots, stems and leaves of K. coccinea. Phenolic acids, flavonoids and lipids in leaves and stems were highly enriched, and several bioactive compounds, such as neochlorogenic acid, chlorogenic acid and quercetin, were highly abundant and have high utilization value.

高漸飛,周瑋,楊艷.基于廣泛靶向代謝組學(xué)分析黑老虎不同部位成分差異[J].熱帶亞熱帶植物學(xué)報(bào),2023,31(3):424~432

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